This week in the lab started off much like it has in the past couple of weeks, with the cells starved of CO2. Even though I had adjusted the incubator's CO2 levels, the CO2 tank decided to become empty. Additionally, JD had some car trouble on Monday, so I had some quality bonding time alone with my cells. While we were making arrangements for the cells to be housed in Cong Li's incubator, I contented myself with observing the culture flasks with the inverted microscope. I was surprised to see the most of the cells actually weren't dead! Three of the flasks still had many adhering cells, and one had enough to seed two new flasks. The petri dish that I had seeded last Friday was also still filled with surviving cells, so I seized the opportunity to dye them with a new concentration of Calcein AM to test with the fluorescence microscope. I think this concentration is a winner:
On Tuesday, I got excited by the brief rainfall and decided to run outside rather than go to the gym with Mel. Sadly, it did not end up raining, so I ended up running through thick, muggy, Kentucky air instead. On the bright side, I discovered Woodland park! I had a good time wandering around and listening to a mini orchestra warming up on the corner stage.
On my way back to Jewell, I found BLACK SWAN BOOKSTORE!! If you'll remember from my first blog post, I found this bookstore on the world wide web in a desperate moment of boredom. I hadn't ended up going yet, because I didn't realize how close it was to where I lived. I plan on visiting before they close today so that I can see if they have Gone With the Wind.
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